Generation Bio is innovating non-viral genetic medicines to provide durable and redosable treatments for hundreds of millions of patients living with rare and prevalent diseases. The company is developing two distinct and complementary platforms: a potent, highly selective cell-targeted lipid nanoparticle (ctLNP) delivery system and a novel immune-quiet DNA (iqDNA) cargo produced by a scalable capsid-free manufacturing process that uses proprietary cell-free rapid enzymatic synthesis (RES). With these platforms, Generation Bio aims to develop the next wave of non-viral genetic medicines to support its mission to extend the reach of genetic medicine to more people living with more diseases, around the world.
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SUMMARY:
Generation Bio seeks an energetic, versatile, and highly motivated Senior Associate Scientist to join our discovery research group. For this position, the successful candidate will support the development of our cell targeted lipid nanoparticle (ctLNP) delivery platform. The ideal candidate has a background in antibody discovery as well as expertise in HTP in vitro, plate-based assay development. They will also have experience with basic protein expression and characterization. They will work with the broader discovery organization to identify as well as characterize structural and functional properties of antibodies and/or related molecules using diverse techniques. This role will be responsible for helping to interrogate novel cell targeting mechanisms and enabling cell specific uptake of our LNPs. Responsibilities will also include cross-functional research to enable early program development for indications in target cell types.
RESPONSIBILITIES:
- Perform a range of biochemical techniques such as ELISA, MSD, Western blotting, SDS-PAGE, and flow cytometry to characterize protein expression and function.
- Perform a range of biophysical techniques such as surface plasmon resonance (SPR), biolayer Interferometry (BLI), size-exclusion chromatography – multiangle light scattering (SEC-MALS), and differential scanning calorimetry (DSC) or fluorimetry (DSF) to characterize protein-ligand interactions and protein stability.
- Assist to analyze and interpret data from biophysical assays to understand binding kinetics, thermodynamics, and binding affinities.
- Assist to troubleshoot and optimize assays as needed to improve sensitivity, specificity, and reproducibility.
- Maintain accurate and up-to-date laboratory records.
- Routinely present updates at group meetings
REQUIREMENTS:
- M.S. or B.S. with a minimum of 2 years of relevant experience, industry experience preferred
- Hands-on experience and knowledge of protein purification using HIC, IEX, SEC and affinity chromatography. Experience with GE AKTA FPLC is preferred.
- Excellent lab technique and the ability to work independently to analyze, interpret, and present data
- Proficiency in biochemical and biophysical characterization methods (e.g. ELISA, DSC, DSF, SDS-PAGE, BLI, SPR, SEC-MALS, flow cytometry) is desired
- Ability to multi-task to support multiple projects across platform discovery organization
- Ability to work in a highly collaborative, team-based environment
- Excellent written and verbal communication skills
Preferred Experience and Skills:
- Experience in developing, optimizing, and validating biophysical and biochemical assays for high-throughput screening applications
- Experience with cell-based assays such as proliferation assays, apoptosis assays, and reporter gene assays.
- Proficient with cell culture manipulation techniques (e.g., transfections, cell-based assays)
- Prior experience with automation/liquid handling systems is a plus
POSITION: Full-Time, Exempt
EEOC Statement: Generation Bio believes in a diverse environment and is committed to equal employment opportunity for all its employees and qualified applicants. We do not discriminate in recruitment, hiring, training, promotion or any other employment practices for reasons of race (to include traits historically associated with race including, but not limited to, hair texture, hair type, hair length, and protective hairstyles such as braids, locks, twists, hair coverings, and Bantu knots), color, gender, religion, national origin, gender identity, age, sexual orientation, marital or veteran status, disability, or any other legally protected status. Generation Bio will make reasonable accommodations for qualified individuals with known disabilities, in accordance with applicable law.
Top Skills
What We Do
Generation Bio is innovating genetic medicines to provide durable, redosable treatments for people living with rare and prevalent diseases. The company’s non-viral platform incorporates a novel DNA construct called closed-ended DNA, or ceDNA; a unique cell-targeted lipid nanoparticle delivery system, or ctLNP; and a highly scalable capsid-free manufacturing process that uses its proprietary cell-free rapid enzymatic synthesis, or RES, to produce ceDNA. The platform is designed to enable multi-year durability from a single dose, to deliver large genetic payloads, including multiple genes, to specific tissues, and to allow titration and redosing to adjust or extend expression levels in each patient. RES has the potential to expand Generation Bio’s manufacturing scale to hundreds of millions of doses to support their mission to extend the reach of genetic medicine to more people, living with more diseases, around the world.
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